Human monocyte-derived macrophages are heterogenous: Proteomic profile of different phenotypes

Human macrophages spontaneously differentiated from adherent monocytes showing two dominant subsets, distinct for morphology (spindle and round) and functions. The aim of this study was to evaluate the intracellular proteome of these two macrophage subsets by means of a microproteomic workflow properly set up to simultaneously identify and quantify proteins from a minimal number of morphotypically heterogeneous cells in culture.