After coronary artery bypass grafting, structural modifications of the saphenous vein wall lead to lumen narrowing in response to the altered hemodynamic conditions. In this paper, the Authors present the design of a novel ex vivo culture system conceived for mimicking central coronary artery hemodynamics, and report the results of biomechanical stimulation experiments using human saphenous vein samples.
The novel pulsatile system uses an aortic-like pressure for forcing a time-dependent coronary-like resistance to obtain the corresponding coronary-like flow rate. The obtained pulsatile pressures and flow rates (diastolic/systolic: 80/120 mmHg and 200/100 mL/min, respectively) showes a reliable mimicking of the complex coronary hemodynamic environment.
The pulsatile system seems a suitable device to identify ex vivo mechanical cues leading to graft adaptation.
- Piola M, Ruiter M, Vismara R, Mastrullo V, Agrifoglio M, Zanobini M, Pesce M, Soncini M, Fiore GB. Full Mimicking of Coronary Hemodynamics for Ex-Vivo Stimulation of Human Saphenous Veins. Ann Biomed Eng 2016 Oct 17. [Epub ahead of print] Go to PubMed